Issue 8, 2000

One-step electropolymeric co-immobilization of glucose oxidase and heparin for amperometric biosensing of glucose

Abstract

Electropolymerization was used for the co-deposition of glucose oxidase and heparin onto metal electrode transducers. Such electropolymeric co-entrapment within a non-conducting poly(1,2-phenylenediamine) (PPD) film imparts both biocatalytic and anticoagulation activities onto the transducer, and greatly improves the performance of the sensor after exposure to whole blood. Essentially identical glucose signals are observed before and after exposure to blood samples. Scanning electron micrographs after such exposure reveal no platelet deposition or formation of a fibrin “clot”. The effect of the heparin co-immobilization on the glucose response is examined. Improved biocompatibility is reported also in connection with a needle-type carbon paste biosensor configuration. The simultaneous localization of the enzyme and heparin offers great promise for simplifying the preparation of enzyme electrodes and designing biocompatible implantable glucose biosensors.

Article information

Article type
Paper
Submitted
19 Jan 2000
Accepted
29 Jun 2000
First published
25 Jul 2000

Analyst, 2000,125, 1431-1434

One-step electropolymeric co-immobilization of glucose oxidase and heparin for amperometric biosensing of glucose

J. Wang, L. Chen, S. B. Hocevar and B. Ogorevc, Analyst, 2000, 125, 1431 DOI: 10.1039/B000550I

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements