Determination of aflatoxin B1 in agricultural commodities by time-resolved fluoroimmunoassay and immunoenzymometric assay

Abstract

A time-resolved fluoroimmunoassay (TR-FIA) and an immunoenzymometric assay (IEMA) were applied for the measurement of aflatoxin B₁ in soya seeds, dried figs and raisins. The extraction procedure was simple and no clean-up was found to be necessary. Limits of detection were 0.5 µg kg^–1 for TR-FIA (range of linearity of calibration graph 2.5–5 × 10⁴ pg; inter-assay relative standard deviation s_r <5%) and 0.2 µg kg^–1 for IEMA (linear range 1.0–5 × 10³ pg; s_r <11%).