Open-source fraction collector for flash column chromatography and continuous flow reactions

Abstract

The design and construction of an open-source fraction collector and its applications in flash column chromatography are described. With the collection vials arranged in a circular array, the outlet of the flash column is guided by an arm to stay above each vial over a defined interval of time, liberating the chemists from the manual labor of swapping vials in high frequency. The assembly is constructed with flow reactor components (plunger pump, tubing, and valves) and optional 3D printed parts (vial holders and arm). The applicability of such a device is demonstrated through relevant examples from the daily work of a chemist, including the purification of crude products, the separation of a reaction mixture, and the fractional collection of effluents from continuous flow reactors.