From the journal:

Chemical Communications

Combining aptamers for thiamphenicol and chloramphenicol for detecting both antibiotics

Yun Shu,ab   Shiyuan Liub  and  Juewen Liu ORCID logo *b  

* Corresponding authors

a School of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou, China

b Department of Chemistry, Waterloo Institute for Nanotechnology, University of Waterloo, Waterloo, Ontario N2L 3G1, Canada
E-mail: liujw@uwaterloo.ca

Abstract

A DNA aptamer named TAP-1 for thiamphenicol (TAP) was selected using the capture-systematic evolution of ligands by exponential enrichment (SELEX) technique, exhibiting a dissociation constant (Kd) of 6.4 μM as determined by isothermal titration calorimetry (ITC). When combined with a previously selected chloramphenicol (CAP) aptamer, they collectively can differentiate TAP and CAP.

Graphical abstract: Combining aptamers for thiamphenicol and chloramphenicol for detecting both antibiotics

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Article information

DOI
https://doi.org/10.1039/D5CC04872A
Article type
Communication
Submitted
24 Aug 2025
Accepted
16 Oct 2025
First published
16 Oct 2025
This article is Open Access
Creative Commons BY-NC license

Chem. Commun., 2025, Advance Article

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Combining aptamers for thiamphenicol and chloramphenicol for detecting both antibiotics

Y. Shu, S. Liu and J. Liu, Chem. Commun., 2025, Advance Article , DOI: 10.1039/D5CC04872A

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