From the journal:

Chemical Communications

A sensitive miRNA detection method based on split-T7 switch modulating CRISPR/Cas12a system

Dayong Li,   Wenting Cheng,   Feifan Yin,   Yanheng Yao,   Zhongyun Wang  and  Yang Xiang  

Abstract

This study presents a novel method for sensitive miRNA detection based on split-T7 switch modulating CRISPR/Cas12a system. By integrating the split-T7 promoter-mediated transcription with CRISPR/Cas12a system, this method can achieve femtomolar detection of the target miRNA within 1 h and successfully analyze miR-21 in samples from various cell lines, demonstrating its potential for clinical applications.

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Article information

DOI
https://doi.org/10.1039/D5CC00170F
Article type
Communication
Submitted
11 Jan 2025
Accepted
19 Feb 2025
First published
19 Feb 2025

Chem. Commun., 2025, Accepted Manuscript

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A sensitive miRNA detection method based on split-T7 switch modulating CRISPR/Cas12a system

D. Li, W. Cheng, F. Yin, Y. Yao, Z. Wang and Y. Xiang, Chem. Commun., 2025, Accepted Manuscript , DOI: 10.1039/D5CC00170F

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