Unravelling microbiome changes in aerobic granular sludge saline wastewater treatment using a slow stepwise salt increase strategy

Abstract

Saline wastewaters mainly result from various industrial activities. In response to water shortage, seawater is increasingly utilized for diverse purposes, leading to an increased production of saline wastewater. The presence of salts in wastewater frequently impairs the efficiency of biological wastewater treatment technologies. Among these, aerobic granular sludge (AGS) has emerged as the most effective aerobic biological treatment process for treating saline wastewater, primarily due to the high biomass aggregation and self-protection afforded by granules. In this study, the AGS biomass was acclimated to saline wastewater through a slow stepwise salt increment strategy over a period of ca. 250 days, from 0 to 14 g NaCl L⁻¹. This acclimation strategy facilitated stable and efficient removal of carbon (>90%), phosphorus (>95%), and ammonium (>98%), without nitrite accumulation in the effluent. Notably, it was observed that the increase in extracellular polymeric substance (EPS) content was concomitant with the enrichment in EPS-producing bacteria, in the AGS biomass. Other salt tolerant bacteria were also enriched in the biomass, particularly those from the Lysobacter and Rhodocyclus bacterial genera, related to nutrient removal and AGS stability. Besides, the high nutrient removal performance was corroborated by the identification of bacteria responsible for these processes. Thus, by employing a slow stepwise increase of wastewater salinity, the AGS process successfully adapted by maintaining the metabolic diversity necessary for various biological removal processes. This study underscores the microbial selection and plasticity inherent in AGS processes, highlighting their significant potential for upgrading saline wastewater treatment.