Efficient bionic nanozyme based on AuPt NPs@ZIF-90 used for cyclic catalysis multimodal tumor therapy

Abstract

Multimodal therapy based on nanozyme is expected to become a novel option for tumor treatment. However, the catalytic efficiency of nanozymes and the hypoxia microenvironment of tumors limit the therapeutic effect of nanozymes. Herein, we screened a small molecule of midazole-2-carboxaldehyde (ICA) to prepare ZIF-90 and embedded gold and platinum nanoparticles to obtain ZAAP. ZAAP possessed a multi-enzymatic cascade of catalytic processes including greatly enhanced peroxidase activity via a “bionic” catalytic microenvironment (enhanced 23-fold), catalase and glucose oxidase activities, resulting in glucose decomposition to continuously supply H₂O₂, peroxidases for the catabolism of H₂O₂ to generate ROS and peroxidase-induced oxygen generation for continuous oxidation of glucose. All the above processes built a catalysis cycle that greatly promotes the generation of ROS and oxygen as well as the consumption of glucose, leading to the chemical dynamic therapy function and alleviating tumor hypoxia. In addition to the photothermal effect of ZAAP, a synergistic treatment of chemical dynamic/photothermal/starvation therapy was achieved, and the tumor inhibition rate reached 96.4% within 2 weeks, indicating that ZAAP shows great potential in nanozyme-based synergistic multimodal tumor treatment.