Unveiling the Dynamic Processes of Dietary Advanced Glycation End-Products (dAGEs) in Absorption, Accumulation, and Gut Microbiota Metabolism

Abstract

This study delves into the dynamics of dietary Advanced Glycation End-products (dAGEs) on host health and gut microbiota. Using 13C-labeled Carboxymethyllysine (CML) bound casein, we identify bound AGEs as the primary entry route, contrasting with free AGEs dominating urinary excretion. Kidneys, not the liver, accumulate dAGEs, emphasizing their pivotal role in health effects. High AGEs (HA) diet prompts rapid gut microbiota changes, with an initial stress-induced mutation phase followed by stabilization. Bacteroides and Parabacteroides emerge as potential dAGEs-utilizing bacteria, influencing microbiota composition. Concurrent metabolic shifts affect lipid and carbohydrate pathways, persisting over time and impacting host metabolic homeostasis. This study illuminates the intricate interplay between dietary AGEs, gut microbiota, and host health, offering insights into the health consequences of short and long-term HA dietary patterns.