Gold nanoparticle-based lateral flow immunoassay for the rapid detection of flutriafol residues in food

Abstract

As a commonly used crop fungicide with proven adverse effects against various organisms, the excessive use of flutriafol (FTF) has resulted in accumulation of a large amount of residues in crops. In this study, a novel hapten was designed by introducing a long interval arm on its benzene ring allowing sufficient exposure of the FTF active site, for the production of a highly sensitive and specific monoclonal antibody with a half-inhibitory concentration of 1.77 ng mL⁻¹. Furthermore, for the first time, a gold nanoparticle-based lateral flow immunoassay (LFIA) was developed for the quantitative detection of FTF in foods with a visual limit of detection in rice, tomatoes, and grapes of 0.5 mg kg⁻¹, 0.2 mg kg⁻¹ and 0.5 mg kg⁻¹, respectively, and a cutoff value of 4 mg kg⁻¹, and a calculated limit of detection of 0.045 mg kg⁻¹, 0.035 mg kg⁻¹ and 0.060 mg kg⁻¹, respectively. The recoveries in spiked samples ranged from 90.2% to 110.1% and these results indicated that the gold nanoparticle-based LFIA developed in this study for the rapid detection of FTF in food is a reliable on-site detection method.