Functional characterization of two efficient glycosyltransferases catalysing the formation of rutin from Sophora japonica L.

Abstract

Two efficient and selective glycosyltransferases were identified from Sophora japonica L. Sj3GT could regio-selectively catalyse 3-O-glucosylation of quercetin to produce isoquercitrin, and Sj6′′RhaT could further catalyse its 6′′-O-rhamnosylation to generate rutin. It is particularly noteworthy that Sj6′′RhaT shows high sugar donor selectivity towards UDP-rhamnose.