Simple manual roller pump-driven valve-free microfluidic solution exchange system for urgent bioassay

Abstract

We introduce a simple-to-use manual roller pump (MRP)-driven and valve-free microfluidic system for sequential solution exchange, followed by a bioassay to detect protein. The polydimethylsiloxane (PDMS)/glass-based disposable device comprises a reaction chamber, multiple micro-flow channels (μFCs), and air vents. The practical solution exchange was realized by sequential injection and withdrawal of several solutions into and from the reaction chamber through constricted μFCs by utilizing changing air pressure of an MRP when a small cylindrical roller was pressed and rolled over a soft silicone tube using a finger. Furthermore, we investigated the effect of surface hydrophobicity on solution exchange. A sandwich fluorescence-based immunoassay to detect human interleukin 2 (IL-2) was performed using this simple microfluidic scheme to demonstrate its suitability for analytical bioassays. The system allowed quick IL-2 detection in 20 min in a pre-functionalized device with a detection limit of 80 pg mL⁻¹ and a range of 125 pg mL⁻¹ to 2.0 ng mL⁻¹. We have thus developed a microfluidic scheme that non-experts can efficiently perform and that can be the fundamental module for low-cost bioassays necessary for emergencies and situations where resources are constrained.