An ultrasensitive electrochemical biosensor for microRNA-21 detection via AuNPs/GAs and Y-shaped DNA dual-signal amplification strategy

Congjuan He; Jiaying Zhao; Yanyi Long; Huisi Yang; Jiangbo Dong; Huan Liu; Zhikun Hu; Mei Yang; Danqun Huo; Changjun Hou

doi:10.1039/D2CC06329H

An ultrasensitive electrochemical biosensor for microRNA-21 detection via AuNPs/GAs and Y-shaped DNA dual-signal amplification strategy†

Congjuan He,‡^a Jiaying Zhao,‡^a Yanyi Long,^a Huisi Yang,

^a Jiangbo Dong,^a Huan Liu,^d Zhikun Hu,^a Mei Yang,

^a Danqun Huo*^ac and Changjun Hou

*^ab

Author affiliations

* Corresponding authors

^a Key Laboratory for Biorheological Science and Technology of Ministry of Education, State and Local Joint Engineering Laboratory for Vascular Implants, Bioengineering College of Chongqing University, Chongqing 400044, P. R. China
E-mail: huodq@cqu.edu.cn, houcj@cqu.edu.cn

^b Chongqing Key Laboratory of Bio-perception & Intelligent Information Processing, School of Microelectronics and Communication Engineering, Chongqing University, Chongqing, P. R. China

^c National Facility for Translational Medicine, Shanghai Jiao Tong University, Shanghai, P. R. China

^d Chongqing Institute for Food and Drug Control, Chongqing 401121, P. R. China

Abstract

Herein, a gold nanoparticles/graphene aerogels (AuNPs/GAs) modified electrochemical biosensor with catalytic hairpin assembly (CHA) and Y-shaped DNA nanostructure dual-signal amplification approaches for ultrasensitive microRNA-21 (miR-21) detection was successfully constructed, which displayed an ultra-wide detection linear range from 5 fM to 50 nM, as well as a relatively low detection limit (LOD) of 14.70 aM (S/N = 3). Furthermore, the sensing strategy had excellent specificity among highly homologous miRNA family members and exhibited satisfactory analytical performance for miRNA detection.

Chemical Communications

An ultrasensitive electrochemical biosensor for microRNA-21 detection via AuNPs/GAs and Y-shaped DNA dual-signal amplification strategy†

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An ultrasensitive electrochemical biosensor for microRNA-21 detection via AuNPs/GAs and Y-shaped DNA dual-signal amplification strategy

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