Issue 61, 2022

Genetic encoding of ε-N-l-lactyllysine for detecting delactylase activity in living cells

Abstract

Lysine ε-N-L-lactylation is a newly discovered post-translational modification. Herein we present the genetic encoding of ε-N-L-lactyllysine in bacterial and mammalian cells, allowing the preparation of site-specifically ε-N-L-lactylated recombinant proteins and the construction of fluorescent and luminescent probes for detecting delactylases in living cells. Using these probes, we demonstrate sirtuin 1 as a potential delactylase for non-histone proteins.

Graphical abstract: Genetic encoding of ε-N-l-lactyllysine for detecting delactylase activity in living cells

Supplementary files

Article information

Article type
Communication
Submitted
09 May 2022
Accepted
01 Jul 2022
First published
01 Jul 2022

Chem. Commun., 2022,58, 8544-8547

Genetic encoding of ε-N-L-lactyllysine for detecting delactylase activity in living cells

Y. Sun, Y. Chen, Y. Xu, Y. Zhang, M. Lu, M. Li, L. Zhou and T. Peng, Chem. Commun., 2022, 58, 8544 DOI: 10.1039/D2CC02643K

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