Issue 53, 2022

Detection of cell membrane proteins using ion-sensitive field effect transistors combined with chemical signal amplification

Abstract

The capture and detection of cells expressing a breast-cancer related membrane protein, namely a BT474 cell line expressing HER2, is demonstrated using ion-sensitive field effect transistors (ISFETs). BT474 cells were exposed to anti-HER2 antibodies and urease-conjugated secondary antibodies to induce chemical signal amplification by adding urea.

Graphical abstract: Detection of cell membrane proteins using ion-sensitive field effect transistors combined with chemical signal amplification

Supplementary files

Article information

Article type
Communication
Submitted
15 Apr 2022
Accepted
25 May 2022
First published
10 Jun 2022
This article is Open Access
Creative Commons BY-NC license

Chem. Commun., 2022,58, 7368-7371

Detection of cell membrane proteins using ion-sensitive field effect transistors combined with chemical signal amplification

M. Tabata, C. Khamhanglit, S. Kotaki and Y. Miyahara, Chem. Commun., 2022, 58, 7368 DOI: 10.1039/D2CC02159E

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