Physicochemical property changes and aroma differences of fermented yellow pea flours: role of Lactobacilli and fermentation time

Abstract

The aim of this study was to evaluate the physicochemical properties and aroma differences of yellow pea flours fermented by five lactic acid bacteria (LAB) strains including two Lactiplantibacillus, two Lactobacillus, and one Lacticaseibacillus with different fermentation time. The cell population and the pH of pea flour slurry, as well as the proximate chemical composition, amino acids, thermal and pasting properties, surface morphology, and aromatic differences of fermented flours were characterized. The cell population of all strains except for Lactobacillus helveticus was observed to reach above 10⁷ CFU mL⁻¹ after 24 h of fermentation. The fermentation with Lactobacilli resulted in the increase of amino acids and ash contents, and the reduction of fat content. Rapid viscosity analysis indicated that short time (18 h) fermentation with L. helveticus drastically improved the pasting properties of the flours by facilitating starch granule expansion. The aromatic compounds of the fermented yellow pea flours were highly reliant on the strains and fermentation time. The untargeted metabolomics analysis with the aid of multivariate data analysis can discriminate the aroma differences among the fermented yellow pea flours. L. acidophilus fermentation led to the production of three aromatic compounds which may contribute to an improved aromatic profile.