Issue 39, 2021

Nanodisc reconstitution of flavin mononucleotide binding domain of cytochrome-P450-reductase enables high-resolution NMR probing

Abstract

Cytochrome-P450-reductase transfers electrons to cytochrome-P450 through its flavin mononucleotide binding domain (FBD). Despite the importance of membrane-anchoring for FBD function, studies have focused on its soluble domain lacking the transmembrane-domain. Here we demonstrate that the reconstitution of FBD in nanodiscs enables high-resolution NMR measurements and renders a stable conformation.

Graphical abstract: Nanodisc reconstitution of flavin mononucleotide binding domain of cytochrome-P450-reductase enables high-resolution NMR probing

Supplementary files

Article information

Article type
Communication
Submitted
22 Feb 2021
Accepted
06 Apr 2021
First published
16 Apr 2021

Chem. Commun., 2021,57, 4819-4822

Nanodisc reconstitution of flavin mononucleotide binding domain of cytochrome-P450-reductase enables high-resolution NMR probing

B. Krishnarjuna, T. Yamazaki, G. M. Anantharamaiah and A. Ramamoorthy, Chem. Commun., 2021, 57, 4819 DOI: 10.1039/D1CC01018B

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