Aptamer-mediated rolling circle amplification for label-free and sensitive detection of histone acetyltransferase activity

Su Jiang; Meng Liu; Wei Tantai; Qinfeng Xu; Xiaoran Zou; Fei Ma; Chun-yang Zhang

doi:10.1039/D0CC07763A

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Aptamer-mediated rolling circle amplification for label-free and sensitive detection of histone acetyltransferase activity†

Su Jiang,‡^a Meng Liu,‡^a Wei Tantai,^b Qinfeng Xu,

^b Xiaoran Zou,^a Fei Ma*^ac and Chun-yang Zhang

*^a

Author affiliations

* Corresponding authors

^a Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Shandong Provincial Key Laboratory of Clean Production of Fine Chemicals, College of Chemistry, Chemical Engineering and Materials Science, Shandong Normal University, Jinan 250014, China
E-mail: cyzhang@sdnu.edu.cn
Fax: +86 0531 82615258
Tel: +86 0531 82615258

^b School of Food and Biological Engineering, Shaanxi University of Science and Technology, Xi’an 710021, China

^c School of Chemistry and Chemical Engineering, Southeast University, Nanjing, China
E-mail: fei@seu.edu.cn

Abstract

We develop for the first time an aptamer-mediated rolling circle amplification approach for label-free and sensitive detection of histone-modifying enzyme (HME) activity. This method can achieve femtomolar sensitivity for histone acetyltransferase Tip60 assay, which is the most sensitive HME assay reported so far. It can be further applied for inhibitor screening, enzyme kinetic analysis, and endogenous Tip60 measurement in cancer cells.

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Article information

DOI: https://doi.org/10.1039/D0CC07763A
Article type: Communication
Submitted: 26 Nov 2020
Accepted: 18 Jan 2021
First published: 18 Jan 2021

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Chem. Commun., 2021,57, 2041-2044

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Aptamer-mediated rolling circle amplification for label-free and sensitive detection of histone acetyltransferase activity

S. Jiang, M. Liu, W. Tantai, Q. Xu, X. Zou, F. Ma and C. Zhang, Chem. Commun., 2021, 57, 2041 DOI: 10.1039/D0CC07763A

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Chemical Communications

Aptamer-mediated rolling circle amplification for label-free and sensitive detection of histone acetyltransferase activity†

Abstract

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Aptamer-mediated rolling circle amplification for label-free and sensitive detection of histone acetyltransferase activity

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