Issue 2, 2021

Colorimetric loop-mediated isothermal amplification (LAMP) for cost-effective and quantitative detection of SARS-CoV-2: the change in color in LAMP-based assays quantitatively correlates with viral copy number

Abstract

We demonstrate a loop-mediated isothermal amplification (LAMP) method to detect and amplify SARS-CoV-2 genetic sequences using a set of in-house designed initiators that target regions encoding the N protein. We were able to detect and amplify SARS-CoV-2 nucleic acids in the range of 62 to 2 × 105 DNA copies by this straightforward method. Using synthetic SARS-CoV-2 samples and RNA extracts from patients, we demonstrate that colorimetric LAMP is a quantitative method comparable in diagnostic performance to RT-qPCR (i.e., sensitivity of 92.85% and specificity of 81.25% in a set of 44 RNA extracts from patients analyzed in a hospital setting).

Graphical abstract: Colorimetric loop-mediated isothermal amplification (LAMP) for cost-effective and quantitative detection of SARS-CoV-2: the change in color in LAMP-based assays quantitatively correlates with viral copy number

Supplementary files

Article information

Article type
Communication
Submitted
01 Sep 2020
Accepted
30 Nov 2020
First published
18 Dec 2020

Anal. Methods, 2021,13, 169-178

Colorimetric loop-mediated isothermal amplification (LAMP) for cost-effective and quantitative detection of SARS-CoV-2: the change in color in LAMP-based assays quantitatively correlates with viral copy number

E. González-González, I. M. Lara-Mayorga, I. P. Rodríguez-Sánchez, Y. S. Zhang, S. O. Martínez-Chapa, G. T. Santiago and M. M. Alvarez, Anal. Methods, 2021, 13, 169 DOI: 10.1039/D0AY01658F

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