A “turn-on” fluorescent sensor for cytosine in aqueous media based on diamino-bridged biphenyl acrylonitrile†
Abstract
The detection of cytosine is of significance for analyzing DNA bases, but no “turn-on” fluorescent sensor for cytosine in aqueous media has been reported so far. In this study, series of diamino-bridged biphenyl acrylonitriles 3a–3c were designed and synthesized with yields of 70–75%. The sensing experiments for 16 types of ions and biomolecules suggested that compound 3b with butanediamino bridges exhibited good selective sensing abilities towards cytosine based on “turn-on” fluorescence enhancement. The detection limit of compound 3b for cytosine attained 1.02 × 10−7 M. The selective sensing ability towards cytosine was little influenced by other species and remained stable between pH = 6–9. The sensing mechanism was proposed by the analysis of Job's plot, 1H NMR and MS binding spectra. The sensing ability for cytosine was successfully used for analysing urine samples with the recovery over 94%. Sample 3b also exhibited good bioimaging performance with bright blue-green fluorescence for sensing cytosine, implying the application prospect in complicated and living environment.