Issue 22, 2019

Photoinduced binding of malachite green copolymer to parallel G-quadruplex DNA

Abstract

Designing ligands that selectively target G-quadruplex DNAs has gained attention due to their possible roles in regulation of gene expression and as anti-cancer agents. In this article, we report irradiation-induced ligand binding to G-quadruplex DNAs which offers a novel approach to targeting specific G-quadruplexes. Photoinduced binding to G-quadruplex DNAs was observed for copolymers of poly(vinyl alcohol) carrying a malachite green moiety (PVAMG). This molecule has an aromatic ring with cationic charge, which after irradiation becomes a binding site for G-quadruplex DNA. PVAMGs acted as neutral polymers with no binding affinity under dark conditions. The photoinduced binding was revealed by fluorescence spectroscopy, NMR spectroscopy, UV melting curve, and DNA polymerase stop assay. PVAMGs showed preference to parallel G-quadruplex structures over mixed parallel/antiparallel structures. PVAMGs were found to be noncytotoxic under both dark and irradiated conditions up to a concentration of 20 μM.

Graphical abstract: Photoinduced binding of malachite green copolymer to parallel G-quadruplex DNA

Supplementary files

Article information

Article type
Paper
Submitted
25 Feb 2019
Accepted
25 Apr 2019
First published
26 Apr 2019

Soft Matter, 2019,15, 4454-4459

Photoinduced binding of malachite green copolymer to parallel G-quadruplex DNA

R. M. Uda, N. Nishimoto, T. Matsui and S. Takagi, Soft Matter, 2019, 15, 4454 DOI: 10.1039/C9SM00411D

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