Endoplasmic reticulum targeted fluorescent probe for the detection of hydrogen sulfide based on a twist-blockage strategy

Abstract

Hydrogen sulfide (H₂S) is very important for humans and is involved in many physiological processes. Here, we designed and reported a new naked-eye colorimetric fluorescent probe Z1 for detecting H₂S in absolute HEPES solution. The fluorescence intensity, after the reaction of the probe and H₂S, is about 32 times that of the probe alone. When the concentration of H₂S is 0–100 μM, the detection limit (DL) is rather low at about 0.15 μM (3σ/slope). The response mechanism is based on the leaving of the 2,4-dinitrobenzene moiety, followed by intramolecular cyclization to give a fluorescent iminocoumarin-benzothiazole group. Moreover, Z1 was applied to endogenous and exogenous H₂S imaging in living cells. The high overlap coefficient proved that probe Z1 has good ER-tracker localization in living cells.