Issue 19, 2019

Enhancement and control of neuron adhesion on polydimethylsiloxane for cell microengineering using a functionalized triblock polymer

Abstract

Polydimethylsiloxane (PDMS)-based neuron microengineering provides new opportunities for spatiotemporal control of neuronal activity and stimuli. The demand for long-lasting adhesive PDMS surfaces has steered the development of straightforward, feasible, and accessible interface modifications. Here, we describe an innovative approach for promoting and engineering neuron adhesion on a PDMS substrate based on a very simple modification using poly-D-lysine-conjugated Pluronic F127, a functionalized triblock polymer. The modification procedure only involves single-step pipetting or microfluidic-guided introduction for the reinforcement of cell adhesion in quantity, extensibility, and stability. Micropatterning at a single-cell resolution, microfluidic long-term culture, and neuron network formation were achieved. The present approach provides a previously unprecedented simple and effective technique for neuron adhesion on PDMS and may be useful for applications in neurobiology, tissue engineering, and neuronal microsystems.

Graphical abstract: Enhancement and control of neuron adhesion on polydimethylsiloxane for cell microengineering using a functionalized triblock polymer

Supplementary files

Article information

Article type
Communication
Submitted
28 Jul 2019
Accepted
23 Aug 2019
First published
23 Aug 2019

Lab Chip, 2019,19, 3162-3167

Enhancement and control of neuron adhesion on polydimethylsiloxane for cell microengineering using a functionalized triblock polymer

W. Liu, K. Han, M. Sun and J. Wang, Lab Chip, 2019, 19, 3162 DOI: 10.1039/C9LC00736A

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