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Visualization of diffusion limited antimicrobial peptide attack on supported lipid membranes

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Abstract

Understanding the mechanism of action of antimicrobial peptides (AMP) is fundamental to the development and design of peptide based antimicrobials. Utilizing fast-scan atomic force microscopy (AFM) we detail the attack of an AMP on both prototypical prokaryotic (DOPC:DOPG) and eukaryotic (DOPC:DOPE) model lipid membranes on the nanoscale and in real time. Previously shown to have a favourable therapeutic index, we study Smp43, an AMP with a helical-hinge-helical topology isolated from the venom of the North African scorpion Scorpio maurus palmatus. We observe the dynamic formation of highly branched defects being supported by 2D diffusion models and further experimental data from liposome leakage assays and quartz crystal microbalance-dissipation (QCM-D) analysis, we propose that Smp43 disrupts these membranes via a common mechanism, which we have termed ‘diffusion limited disruption’ that encompasses elements of both the carpet model and the expanding pore mechanism.

Graphical abstract: Visualization of diffusion limited antimicrobial peptide attack on supported lipid membranes

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Publication details

The article was received on 05 Apr 2018, accepted on 18 May 2018 and first published on 12 Jul 2018


Article type: Paper
DOI: 10.1039/C8SM00707A
Citation: Soft Matter, 2018, Advance Article
  • Open access: Creative Commons BY-NC license
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    Visualization of diffusion limited antimicrobial peptide attack on supported lipid membranes

    G. R. Heath, P. L. Harrison, P. N. Strong, S. D. Evans and K. Miller, Soft Matter, 2018, Advance Article , DOI: 10.1039/C8SM00707A

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