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MODELING OF DNA TRANSPORT IN VISCOELASTIC ELECTRO-HYDRODYNAMIC FLOWS FOR ENHANCED SIZE SEPARATION

Abstract

DNA separation and analysis has advanced over the last years benefiting from microfluidic systems that reduce sample volumes and analysis costs, essential for sequencing and disease identification in body fluids. We recently developed the µLAS technology that enables the separation, concentration, and analysis of nucleic acids with high sensitivity. The technology combines a hydrodynamic flow actuation and an opposite electrophoretic force in viscoelastic polymer solutions. Combining hydrodynamics first principles and statistical mechanics, we provide, in this paper, a quantitative model of DNA transport capable of predicting the device performance with the exclusive use of one adjustable parameter associated to the amplitude of transverse viscoelastic forces. The model proves to be in remarkable agreement with DNA separation experiments, and allows us to define optimal conditions that result in a maximal resolution length of 7 bp. We finally discuss the usefulness of our model for separation technologies involving viscoelastic liquids.

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Publication details

The article was received on 23 Mar 2018, accepted on 17 May 2018 and first published on 17 May 2018


Article type: Paper
DOI: 10.1039/C8SM00611C
Citation: Soft Matter, 2018, Accepted Manuscript
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    MODELING OF DNA TRANSPORT IN VISCOELASTIC ELECTRO-HYDRODYNAMIC FLOWS FOR ENHANCED SIZE SEPARATION

    B. Chami, M. Socol, M. Manghi and A. Bancaud, Soft Matter, 2018, Accepted Manuscript , DOI: 10.1039/C8SM00611C

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