Jump to main content
Jump to site search


Product-oriented chemical surface modification of a levansucrase (SacB) via an ene-type reaction

Author affiliations

Abstract

Carbohydrate processing enzymes are sophisticated tools of living systems that have evolved to execute specific reactions on sugars. Here we present for the first time the site-selective chemical modification of exposed tyrosine residues in SacB, a levansucrase from Bacillus megaterium (Bm-LS) for enzyme engineering purposes via an ene-type reaction. Bm-LS is unable to sustain the synthesis of high molecular weight (HMW) levan (a fructose polymer) due to protein–oligosaccharide dissociation events occurring at an early stage during polymer elongation. We switched the catalyst from levan-like oligosaccharide synthesis to the efficient production of a HMW fructan polymer through the covalent addition of a flexible chemical side-chain that fluctuates over the central binding cavity of the enzyme preventing premature oligosaccharide disengagement.

Graphical abstract: Product-oriented chemical surface modification of a levansucrase (SacB) via an ene-type reaction

Back to tab navigation

Supplementary files

Publication details

The article was received on 16 Mar 2018, accepted on 18 May 2018 and first published on 22 May 2018


Article type: Edge Article
DOI: 10.1039/C8SC01244J
Citation: Chem. Sci., 2018, Advance Article
  • Open access: Creative Commons BY-NC license
  •   Request permissions

    Product-oriented chemical surface modification of a levansucrase (SacB) via an ene-type reaction

    M. E. Ortiz-Soto, J. Ertl, J. Mut, J. Adelmann, T. A. Le, J. Shan, J. Teßmar, A. Schlosser, B. Engels and J. Seibel, Chem. Sci., 2018, Advance Article , DOI: 10.1039/C8SC01244J

    This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence. Material from this article can be used in other publications provided that the correct acknowledgement is given with the reproduced material and it is not used for commercial purposes.

    Reproduced material should be attributed as follows:

    • For reproduction of material from NJC:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the Centre National de la Recherche Scientifique (CNRS) and the RSC.
    • For reproduction of material from PCCP:
      [Original citation] - Published by the PCCP Owner Societies.
    • For reproduction of material from PPS:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the European Society for Photobiology, the European Photochemistry Association, and RSC.
    • For reproduction of material from all other RSC journals:
      [Original citation] - Published by The Royal Society of Chemistry.

    Information about reproducing material from RSC articles with different licences is available on our Permission Requests page.

Search articles by author

Spotlight

Advertisements