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Issue 1, 2018
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Rapid, selective and stable HaloTag-LbADH immobilization directly from crude cell extract for the continuous biocatalytic production of chiral alcohols and epoxides

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Abstract

A strategy for biocatalyst immobilization in flow directly from the crude cell extract is described. The efficiency and the stability of the immobilized enzyme were demonstrated during the asymmetric reduction of a range of ketones. The cascade two-step chemo-enzymatic preparation of chiral epoxides was possible through the initial ketone bioreduction to an intermediate halohydrin followed by its intramolecular cyclization.

Graphical abstract: Rapid, selective and stable HaloTag-LbADH immobilization directly from crude cell extract for the continuous biocatalytic production of chiral alcohols and epoxides

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Publication details

The article was received on 16 Oct 2017, accepted on 07 Dec 2017 and first published on 17 Jan 2018


Article type: Communication
DOI: 10.1039/C7RE00173H
Citation: React. Chem. Eng., 2018,3, 8-12
  • Open access: Creative Commons BY license
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    Rapid, selective and stable HaloTag-LbADH immobilization directly from crude cell extract for the continuous biocatalytic production of chiral alcohols and epoxides

    J. Döbber, M. Pohl, S. V. Ley and B. Musio, React. Chem. Eng., 2018, 3, 8
    DOI: 10.1039/C7RE00173H

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