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Issue 6, 2018
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Ir(III) complex-based oxygen imaging of living cells and ocular fundus with a gated ICCD camera

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Abstract

Phosphorescence lifetime imaging methods using oxygen-sensitive probes are very useful for visualizing the oxygen status of living cells and tissues with high spatial resolution. We aim to develop a useful oxygen detection technique combining a phosphorescent oxygen probe and an optimal detection method. Herein we present a biological oxygen imaging method using a microscope equipped with a gated intensified charge-coupled device (ICCD) camera as a detector and an Ir(III) complex as a phosphorescent oxygen probe. Microscopic luminescence images of monolayer HT-29 cells (human colorectal adenocarcinoma cells) obtained using the cell-penetrating Ir(III) complex BTPDM1 and an inverted microscope demonstrated that this method allowed visualization of the oxygen gradient produced in a monolayer of cultured cells when the monolayer is covered with a thin coverslip. Furthermore, combining the IR-emitting Ir(III) complex DTTPH-PEG24 with a macrozoom microscope equipped with a gated ICCD camera enabled both the visualization of retinal vessels near the optic disc and the monitoring of oxygen level changes in a rabbit retina upon changing the inhaled oxygen content.

Graphical abstract: Ir(iii) complex-based oxygen imaging of living cells and ocular fundus with a gated ICCD camera

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Publication details

The article was received on 22 Mar 2018, accepted on 20 May 2018 and first published on 29 May 2018


Article type: Paper
DOI: 10.1039/C8PP00122G
Citation: Photochem. Photobiol. Sci., 2018,17, 846-853
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    Ir(III) complex-based oxygen imaging of living cells and ocular fundus with a gated ICCD camera

    H. Akiyama, I. Takahashi, Y. Shimoda, R. Mukai, T. Yoshihara and S. Tobita, Photochem. Photobiol. Sci., 2018, 17, 846
    DOI: 10.1039/C8PP00122G

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