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Selective sensing of nitric oxide by a 9,10-phenanthroquinone–pyridoxal based fluorophore

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Abstract

In this article, we have designed and synthesized a new, convenient and efficient phenanthroquinone–pyridoxal based fluorogenic probe PQPY, highly suitable for the selective and sensitive detection of nitric oxide in an aerated aqueous (7 : 3/H2O : MeCN) medium at pH 7.0 (10 mM HEPES buffer). Upon addition of nitric oxide, this probe exhibits emission in the green region (λem = 505 nm) which is ascribed to ICT (intramolecular charge transfer) from the phenanthroquinone moiety to the imidazole –N–N[double bond, length as m-dash]O fragment. The apparent formation constant, Kf, of the NO product of the ligand is (1.00 ± 0.2) × 105 M−1 and the LOD is 78 nM. The substantial enhancement of the life-time of the ligand (τ0 = 2.68 ns) occurs due to binding with nitric oxide (τ0 = 3.96 ns). This probe is low cytotoxicity, cell permeable and suitable for living cell imaging application.

Graphical abstract: Selective sensing of nitric oxide by a 9,10-phenanthroquinone–pyridoxal based fluorophore

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Publication details

The article was received on 19 Mar 2018, accepted on 19 Jul 2018 and first published on 21 Jul 2018


Article type: Paper
DOI: 10.1039/C8PP00115D
Citation: Photochem. Photobiol. Sci., 2018, Advance Article
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    Selective sensing of nitric oxide by a 9,10-phenanthroquinone–pyridoxal based fluorophore

    D. Maiti, A. S. M. Islam, M. Sasmal, C. Prodhan and M. Ali, Photochem. Photobiol. Sci., 2018, Advance Article , DOI: 10.1039/C8PP00115D

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