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Disassembly-driven Signal Turn-on Probes for Bimodal Detection of DNA with 19F NMR and Fluorescence

Abstract

To image DNAs in vivo, molecular probes that can detect DNA with a highly transparent signal are required. In this study, we designed and synthesized amphiphilic molecular probes that can detect DNA with a highly transparent 19F NMR signal with a signal turn-on manner based on the self-assembly and target-dependent-disassembly of the probe molecules in aqueous solution. Fluorescence and dynamic light scattering study revealed that these amphiphilic probes consisting of bisbenzimide H 33258 and 3,5-bis(trifluoromethyl)benzene are assembled and form aggregate in aqueous solution, and that the aggregates are collapsed when the probes bind to double-stranded DNA via selective interaction between bisbenzimide H 33258 moiety and AATT double-stranded region of DNA. Since the target-dependent-disassembly of the probes caused turn-on of 19F NMR and fluorescence signal, bimodal detection of double-stranded DNA was successfully achieved.

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Publication details

The article was received on 08 Sep 2018, accepted on 13 Sep 2018 and first published on 14 Sep 2018


Article type: Paper
DOI: 10.1039/C8OB02218F
Citation: Org. Biomol. Chem., 2018, Accepted Manuscript
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    Disassembly-driven Signal Turn-on Probes for Bimodal Detection of DNA with 19F NMR and Fluorescence

    T. Sakamoto, D. Hasegawa and K. Fujimoto, Org. Biomol. Chem., 2018, Accepted Manuscript , DOI: 10.1039/C8OB02218F

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