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Issue 24, 2018
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Development of oligonucleotide-based antagonists of Ebola virus protein 24 inhibiting its interaction with karyopherin alpha 1

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Abstract

The investigation of protein–protein interactions (PPIs) and the preparation of antagonists are important for determining whether certain proteins are suitable medical targets. In the present study, we used the capillary electrophoresis-systematic evolution of ligands by exponential enrichment to generate natural and artificial nucleic acid aptamers targeting Ebola virus protein 24 (eVP24), demonstrating that artificial aptamers, synthesised utilising a uridine analogue with an adenine residue at its C5 position, exhibited activities exceeding those of natural ones. To confirm the functionality of the as-prepared aptamers, their abilities to inhibit the PPIs of eVP24 were determined by capillary electrophoresis and bio-layer interferometry, and the obtained results unambiguously demonstrated that these aptamers interacted with the functional site of eVP24 and were thus good antagonists.

Graphical abstract: Development of oligonucleotide-based antagonists of Ebola virus protein 24 inhibiting its interaction with karyopherin alpha 1

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Publication details

The article was received on 23 Mar 2018, accepted on 24 May 2018 and first published on 31 May 2018


Article type: Paper
DOI: 10.1039/C8OB00706C
Citation: Org. Biomol. Chem., 2018,16, 4456-4463
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    Development of oligonucleotide-based antagonists of Ebola virus protein 24 inhibiting its interaction with karyopherin alpha 1

    K. Tanaka, Y. Kasahara, Y. Miyamoto, O. Takumi, T. Kasai, K. Onodera, M. Kuwahara, M. Oka, Y. Yoneda and S. Obika, Org. Biomol. Chem., 2018, 16, 4456
    DOI: 10.1039/C8OB00706C

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