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Issue 19, 2018
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Chemo-enzymatic synthesis of isotopically labeled nicotinamide riboside

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Abstract

As a cofactor for numerous reactions, NAD+ is found widely dispersed across many maps of cellular metabolism. This core redox role alone makes the biosynthesis of NAD+ of great interest. Recent studies have revealed new biological roles for NAD+ as a substrate for diverse enzymes that regulate a broad spectrum of key cellular tasks. These NAD+-consuming enzymes further highlight the importance of understanding NAD+ biosynthetic pathways. In this study, we developed a chemo-enzymatic synthesis of isotopically labeled NAD+ precursor, nicotinamide riboside (NR). The synthesis of NR isotopomers allowed us to unambiguously determine that NR is efficiently converted to NAD+ in the cellular environment independent of degradation to nicotinamide, and it is incorporated into NAD+ in its intact form. The versatile synthetic method along with the isotopically labeled NRs will provide powerful tools to further decipher the important yet complicated NAD+ metabolism.

Graphical abstract: Chemo-enzymatic synthesis of isotopically labeled nicotinamide riboside

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Publication details

The article was received on 05 Mar 2018, accepted on 23 Apr 2018 and first published on 23 Apr 2018


Article type: Paper
DOI: 10.1039/C8OB00552D
Citation: Org. Biomol. Chem., 2018,16, 3662-3671
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    Chemo-enzymatic synthesis of isotopically labeled nicotinamide riboside

    A. Tran, R. Yokose and Y. Cen, Org. Biomol. Chem., 2018, 16, 3662
    DOI: 10.1039/C8OB00552D

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