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Issue 20, 2018
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Time-lapse monitoring of TLR2 ligand internalization with newly developed fluorescent probes

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Abstract

As a mammalian toll-like receptor family member protein, TLR2 recognizes lipoproteins from bacteria and modulates the immune response by inducing the expression of various cytokines. We have developed fluorescence-labeled TLR2 ligands with either hydrophilic or hydrophobic fluorescence groups. The labeled ligands maintained the inflammatory IL-6 induction activity and enabled us to observe the internalization and colocalization of the TLR2 ligands using live-cell imaging. The time-lapse monitoring in the live-cell imaging of the fluorescence-labeled TLR2 ligand showed that TLR2/CD14 expression in the host cells enhanced the internalization of TLR2 ligand molecules.

Graphical abstract: Time-lapse monitoring of TLR2 ligand internalization with newly developed fluorescent probes

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Publication details

The article was received on 30 Dec 2017, accepted on 02 May 2018 and first published on 05 May 2018


Article type: Paper
DOI: 10.1039/C7OB03205F
Citation: Org. Biomol. Chem., 2018,16, 3824-3830
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    Time-lapse monitoring of TLR2 ligand internalization with newly developed fluorescent probes

    Y. Arai, K. Yokoyama, Y. Kawahara, Q. Feng, I. Ohta, A. Shimoyama, S. Inuki, K. Fukase, K. Kabayama and Y. Fujimoto, Org. Biomol. Chem., 2018, 16, 3824
    DOI: 10.1039/C7OB03205F

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