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Single-stranded templates as railroad tracks for hierarchical assembly of DNA origami

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Abstract

DNA origami is one of the most effective tools for bottom-up construction of novel objects and devices at the nanometer-scale. However, many applications require larger structures than can be obtained with the conventional single-stranded scaffold, typically 7249 nucleotides. Here, we address this limitation by developing custom-made single-stranded scaffolds that bind pre-assembled origami tiles and induce their one-dimensional organization in high yields. Our synthetic method allows the conversion of multiple repetitive and unique sequences into correctly assembled, large backbones, and to finely tune the position and frequency of each building block. Granted with these regions, three and five origami tiles were successfully arranged in 1-D with the aid of one or two scaffolds, forming a nano-“railroad track”. This new method increases length scale in DNA origami without increasing cost and complexity, and is anticipated to increase the yield of other approaches aiming to assemble large origami structures.

Graphical abstract: Single-stranded templates as railroad tracks for hierarchical assembly of DNA origami

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Publication details

The article was received on 18 Apr 2018, accepted on 24 Jun 2018 and first published on 29 Jun 2018


Article type: Paper
DOI: 10.1039/C8NR03185A
Citation: Nanoscale, 2018, Advance Article
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    Single-stranded templates as railroad tracks for hierarchical assembly of DNA origami

    J. F. Rahbani, J. C. C. Hsu, P. Chidchob and H. F. Sleiman, Nanoscale, 2018, Advance Article , DOI: 10.1039/C8NR03185A

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