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Issue 19, 2018
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Sticky-flares for in situ monitoring of human telomerase RNA in living cells

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Abstract

Human telomerase RNA (hTR), a template of telomerase for telomeric repeat synthesis, was used to reflect the telomerase activity and act as a potential target of antitumor therapy. Here, we report a novel DNA-conjugated AuNP probe termed sticky-flares for the in situ detection of intracellular human telomerase RNA. The sticky-flares probe is capable of entering living cells directly without any auxiliary and recognizing the binding domain of human telomerase RNA. On recognition, the fluorophore-modified recognition flares can specifically bind to the target, separate from the sticky-flares and act as a fluorescent reporter to quantify and dynamically profile human telomerase RNA in living cells. We envision that the sticky-flares probe would be a valuable platform to investigate the function and regulation of hTR in antitumor therapy and hTR-related drug invention.

Graphical abstract: Sticky-flares for in situ monitoring of human telomerase RNA in living cells

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Publication details

The article was received on 11 Feb 2018, accepted on 16 Apr 2018 and first published on 17 Apr 2018


Article type: Paper
DOI: 10.1039/C8NR01260A
Citation: Nanoscale, 2018,10, 9386-9392
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    Sticky-flares for in situ monitoring of human telomerase RNA in living cells

    Q. Wu, Z. Liu, L. Su, G. Han, R. Liu, J. Zhao, T. Zhao, C. Jiang and Z. Zhang, Nanoscale, 2018, 10, 9386
    DOI: 10.1039/C8NR01260A

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