Highly specific targeting of human acute myeloid leukaemia cells using pharmacologically active nanoconjugates

Inna M. Yasinska; Giacomo Ceccone; Isaac Ojea-Jimenez; Jessica Ponti; Rohanah Hussain; Giuliano Siligardi; Steffen M. Berger; Elizaveta Fasler-Kan; Marco Bardelli; Luca Varani; Walter Fiedler; Jasmin Wellbrock; Ulrike Raap; Bernhard F. Gibbs; Luigi Calzolai; Vadim V. Sumbayev

doi:10.1039/C7NR09436A

Highly specific targeting of human acute myeloid leukaemia cells using pharmacologically active nanoconjugates†

Inna M. Yasinska,*^a Giacomo Ceccone,^b Isaac Ojea-Jimenez,^b Jessica Ponti,^b Rohanah Hussain,^c Giuliano Siligardi,^c Steffen M. Berger,^d Elizaveta Fasler-Kan,^d Marco Bardelli,^e Luca Varani,^e Walter Fiedler,^f Jasmin Wellbrock,^f Ulrike Raap,^g Bernhard F. Gibbs,*^ag Luigi Calzolai*^b and Vadim V. Sumbayev*^a

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^a School of Pharmacy, University of Kent, Anson Building, Central Avenue, Chatham Maritime, Kent, UK
E-mail: I.Yasinska-24@kent.ac.uk, V.Sumbayev@kent.ac.uk, bernhard.gibbs@uni-oldenburg.de

^b European Commission Joint Research Centre, Ispra, Italy
E-mail: luigi.calzolai@ec.europa.eu

^c Beamline B23, Diamond Light Source, Didcot, UK

^d Department of Paediatric Surgery and Department of Biomedical Research, Children's Hospital, Inselspital, University of Bern, 3010 Bern, Switzerland

^e Institute for Research in Biomedicine, Universita’ della Svizzera italiana (USI), Via Vela 6, 6500 Bellinzona, Switzerland

^f Department of Oncology, Hematology and Bone Marrow Transplantation with Section Pneumology, Hubertus Wald University Cancer Center, University Medical Center Hamburg-Eppendorf, Germany

^g Department of Dermatology, University of Oldenburg, Germany

Abstract

In this study we used 5 nm gold nanoparticles as delivery platforms to target cancer cells expressing the immune receptor Tim-3 using single chain antibodies. Gold surfaces were also covered with the cytotoxic drug rapamycin which was immobilised using a glutathione linker. These nanoconjugates allowed highly specific and efficient delivery of cytotoxic rapamycin into human malignant blood cells.

Nanoscale

Highly specific targeting of human acute myeloid leukaemia cells using pharmacologically active nanoconjugates†

Abstract

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Highly specific targeting of human acute myeloid leukaemia cells using pharmacologically active nanoconjugates

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