Polydiacetylenic nanofibers as new siRNA vehicles for in vitro and in vivo delivery

P. Neuberg; I. Hamaidi; S. Danilin; M. Ripoll; V. Lindner; M. Nothisen; A. Wagner; A. Kichler; T. Massfelder; J.-S. Remy

doi:10.1039/C7NR09202D

Polydiacetylenic nanofibers as new siRNA vehicles for in vitro and in vivo delivery†

P. Neuberg,

^a I. Hamaidi,^b S. Danilin,^b M. Ripoll,^a V. Lindner,^c M. Nothisen,^a A. Wagner,

^d A. Kichler,

^a T. Massfelder

*^b and J.-S. Remy

*^a

Author affiliations

* Corresponding authors

^a V-SAT Laboratory, Vectors: Synthesis and Therapeutic Applications, Labex Medalis, CAMB UMR7199 CNRS-Université de Strasbourg, Faculty of Pharmacy, Illkirch, France
E-mail: remy@unistra.fr

^b Laboratory “Voies de signalisation du développement et du stress cellulaire dans les cancers digestifs et urologiques” (VSDS), Team 3 “Signalisation et communication cellulaires dans les cancers du rein et de la prostate” - UMR_S 1113, INSERM-Université de Strasbourg, France
E-mail: massfeld@unistra.fr

^c Hôpitaux universitaires de Strasbourg, Hôpital de Strasbourg-Hautepierre, Department of Pathology, Strasbourg, France

^d Laboratory of Bio-FunctionalChemistry (BFC), Labex Medalis, CAMB UMR7199 CNRS-Université de Strasbourg, Faculty of Pharmacy, Illkirch, France

Abstract

Polydiacetylenic nanofibers (PDA-Nfs) obtained by photopolymerization of surfactant 1 were optimized for intracellular delivery of small interfering RNAs (siRNAs). PDA-Nfs/siRNA complexes efficiently silenced the oncogene Lim-1 in the renal cancer cells 786-O in vitro. Intraperitoneal injection of PDA-Nfs/siLim1 downregulated Lim-1 in subcutaneous tumor xenografts obtained with 786-O cells in nude mice. Thus, PDA-Nfs represent an innovative system for in vivo delivery of siRNAs.

Nanoscale

Polydiacetylenic nanofibers as new siRNA vehicles for in vitro and in vivo delivery†

Abstract

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Polydiacetylenic nanofibers as new siRNA vehicles for in vitro and in vivo delivery

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