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Horseradish peroxidase-mediated in situ synthesis of silver nanoparticles: Application for Hg2+ sensing

Abstract

An indirect enzyme-induced in situ formation of silver nanoparticles (AgNPs) was investigated for the spectrophotometric detection of inorganic mercury (Hg2+) in aqueous solution. The enzymatic activity of horseradish peroxidase (HRP) assists the formation of AgNPs, which is prevented upon the addition of excess amount of hydrogen peroxide (H2O2). On addition of enzyme substrate 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), the excess amount of H2O2 converts the ABTS into radical cation (ABTS•+), which could help to restart the HRP activity for formation of AgNPs. The addition of increasing Hg2+ (HRP inhibitor) in the reaction mixture resulted in progressive inhibition of the enzyme activity and could cause the AgNP accumulation. The morphological changes caused during the enzyme-induced formation of AgNPs were further studied by transmission electron microscopy (TEM). The developed probe achieved good limit of detection 0.78×10‒9 M and limit of quantification 2.6×10‒9 M in aqueous solution. The probe also demonstrated high recovery percentages for Hg2+ spiked in environmental matrices highlighting the importance of the developed probe.

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Publication details

The article was received on 28 Apr 2018, accepted on 12 Jul 2018 and first published on 13 Jul 2018


Article type: Paper
DOI: 10.1039/C8NJ02083C
Citation: New J. Chem., 2018, Accepted Manuscript
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    Horseradish peroxidase-mediated in situ synthesis of silver nanoparticles: Application for Hg2+ sensing

    N. K. D, N. Chandrasekaran and A. Mukherjee, New J. Chem., 2018, Accepted Manuscript , DOI: 10.1039/C8NJ02083C

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