Jump to main content
Jump to site search


Metal–organic framework nanomaterial as an ideal loading platform for ultrasensitive electrochemiluminescence immunoassay

Abstract

An ultrasensitive sandwich-type electrochemiluminescent (ECL) immunosensor suitable for identifying carcinoembryonic antigens (CEAs) was developed, using a metal–organic framework nanomaterial (AuNPs@NPCGO) as an ideal loading platform and Ru(phen)32+-doped silica nanoparticles (RuSiNPs) as an ECL signaling unit. The AuNPs@NPCGO nanocomposites were synthesized via in-situ deposition of AuNPs on NPCGO as this configuration exhibits high conductivity, strong fixed ability, and excellent stability. The use of RuSiNPs as signaling units provided numerous luminophores that enhanced the ECL signals. Besides the aminated RuSiNPs nanoparticles, which had a large specific surface area and good biocompatibility, functioned as superior matrix for immobilizing numerous secondary antibodies (Ab2). The proposed ECL immunosensor demonstrated excellent performance for CEA detection in concentrations of 0.01 ng mL-1 to 80 ng mL-1 with the detection limit of 0.003 ng mL-1 at the signal-to-noise ratio of three.

Back to tab navigation

Publication details

The article was received on 26 Apr 2018, accepted on 06 Jul 2018 and first published on 13 Jul 2018


Article type: Paper
DOI: 10.1039/C8NJ02038H
Citation: New J. Chem., 2018, Accepted Manuscript
  •   Request permissions

    Metal–organic framework nanomaterial as an ideal loading platform for ultrasensitive electrochemiluminescence immunoassay

    X. Huang, X. Deng, W. Qi and D. Wu, New J. Chem., 2018, Accepted Manuscript , DOI: 10.1039/C8NJ02038H

Search articles by author

Spotlight

Advertisements