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Fluorescent dual aptasensor for rapid and sensitive onsite detection of E.coli O157:H7 and its validation onto various food matrices

Abstract

The brisk analysis of food products remains a keen area of concern and to redress such issues, rapid, highly efficient as well robust on-site detection platforms are essential. Escherichia coli O157:H7 remains most frequently encountered organism associated with food borne illness globally. The present study focus on rapid onsite E.coli O157:H7 detection platform development. To accomplish this, target specific ssDNA aptamers was generated by whole cell SELEX, wherein high reactive ones (EcoR1 and EcoR2) with least dissociated constant (Kd) values were further biotin tagged (EcoR1) as well as fluorescent labelled (EcoR2). Forwith, biotin labelled aptamers immobilized onto silane-glutaraldehyde functionalized glass slides acts as capturing ligands and QDs labelled aptamers as revealing probes The detection limit of the platform was found to be 102 CFU/mL within 15 mins duration and no cross-reactivity towards other related pathogens were observed. The developed assay was further evaluated onto complex food matrices for the presence of E.coli O157:H7 whereupon the results were found to be consistent with conventional methods with a recovery percentage between 76% to 91%. In conclusion, the developed method may find a better utility in real time food samples monitoring.

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Publication details

The article was received on 01 Mar 2018, accepted on 14 May 2018 and first published on 15 May 2018


Article type: Paper
DOI: 10.1039/C8NJ00997J
Citation: New J. Chem., 2018, Accepted Manuscript
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    Fluorescent dual aptasensor for rapid and sensitive onsite detection of E.coli O157:H7 and its validation onto various food matrices

    R. RM, A. Jayakrishnan, C. H. R, V. M and K. Kadirvelu, New J. Chem., 2018, Accepted Manuscript , DOI: 10.1039/C8NJ00997J

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