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Issue 12, 2018
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Determination of baclofen and vigabatrin by microchip electrophoresis with fluorescence detection: application of field-enhanced sample stacking and dynamic pH junction

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Abstract

A simple, rapid and sensitive microchip electrophoretic (MCE) method with fluorescence detection is described for the simultaneous determination of two GABA analogue drugs, baclofen (BCN) and vigabatrin (VGN). Pre-microchip derivatization of both analytes with 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F) was performed in a basic borate buffer. The NBD-fluorescent derivatives of the studied drugs (λex/em 470/540) were baseline separated in a dynamically-coated poly(methyl methacrylate) microfluidic channel within 120 s using a 40 mM borate buffer containing 0.4% methylcellulose as the background solution. The ability of methylcellulose to form a network sieve of small pore size allowed the labelled analytes to be separated efficiently according to their molecular size variations with a resolution factor equal to 7.8 and a number of theoretical plates of more than 500 000 per meter. The MCE method was applied to assay BCN and VGN in tablets using 6-aminohexanoic acid as an internal standard. The method sensitivity was enhanced by application of combined stacking and a dynamic pH junction. The method was applied to assay BCN in human plasma and human urine samples with a detection limit lower than 0.3 ng mL−1 and mean extraction recoveries of more than 95% (% RSD < 7) after protein precipitation with methanol.

Graphical abstract: Determination of baclofen and vigabatrin by microchip electrophoresis with fluorescence detection: application of field-enhanced sample stacking and dynamic pH junction

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Publication details

The article was received on 20 Feb 2018, accepted on 01 May 2018 and first published on 09 May 2018


Article type: Paper
DOI: 10.1039/C8NJ00829A
Citation: New J. Chem., 2018,42, 9965-9974
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    Determination of baclofen and vigabatrin by microchip electrophoresis with fluorescence detection: application of field-enhanced sample stacking and dynamic pH junction

    A. M. Zeid, N. Kaji, J. J. M. Nasr, F. Belal, M. I. Walash and Y. Baba, New J. Chem., 2018, 42, 9965
    DOI: 10.1039/C8NJ00829A

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