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High-throughput single-molecule bioassay using micro-reactor arrays with a concentration gradient of target molecules

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Abstract

Micro-reactor arrays enable highly sensitive and quantitative bioassays at a single-molecule level. Accordingly, they are widely used for sensitive “digital” bioassays, e.g., digital PCR and digital ELISA. Despite high integration, individual reactors in digital bioassays are filled with a uniform reaction solution, thus limiting the ability to simultaneously conduct multiple bioassays under different conditions using integrated reactors in parallel, resulting in the loss of potential throughput. We developed micro-reactor arrays with a concentration gradient of target molecules, in which individual reactors sealed with a lipid-bilayer membrane contained a precise amount of target molecules. Using the arrays, we successfully demonstrated multiple single-molecule bioassays in parallel using alkaline phosphatase or α-hemolysin, key components in various biomedical sensors. This new platform extends the versatility of micro-reactor arrays and could enable further analytical and pharmacological applications.

Graphical abstract: High-throughput single-molecule bioassay using micro-reactor arrays with a concentration gradient of target molecules

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Publication details

The article was received on 28 May 2018, accepted on 29 Jun 2018 and first published on 09 Aug 2018


Article type: Paper
DOI: 10.1039/C8LC00535D
Citation: Lab Chip, 2018, Advance Article
  • Open access: Creative Commons BY license
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    High-throughput single-molecule bioassay using micro-reactor arrays with a concentration gradient of target molecules

    R. Watanabe, T. Komatsu, S. Sakamoto, Y. Urano and H. Noji, Lab Chip, 2018, Advance Article , DOI: 10.1039/C8LC00535D

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