Issue 17, 2018

A flow focusing microfluidic device with an integrated Coulter particle counter for production, counting and size characterization of monodisperse microbubbles

Abstract

Flow focusing microfluidic devices (FFMDs) have been investigated for the production of monodisperse populations of microbubbles for chemical, biomedical and mechanical engineering applications. High-speed optical microscopy is commonly used to monitor FFMD microbubble production parameters, such as diameter and production rate, but this limits the scalability and portability of the approach. In this work, a novel FFMD design featuring integrated electronics for measuring microbubble diameters and production rates is presented. A micro Coulter particle counter (μCPC), using electrodes integrated within the expanding nozzle of an FFMD (FFMD-μCPC), was designed, fabricated and tested. Finite element analysis (FEA) of optimal electrode geometry was performed and validated with experimental data. Electrical data was collected for 8–20 μm diameter microbubbles at production rates up to 3.25 × 105 MB s−1 and compared to both high-speed microscopy data and FEA simulations. Within a valid operating regime, Coulter counts of microbubble production rates matched optical reference values. The Coulter method agreed with the optical reference method in evaluating the microbubble diameter to a coefficient of determination of R2 = 0.91.

Graphical abstract: A flow focusing microfluidic device with an integrated Coulter particle counter for production, counting and size characterization of monodisperse microbubbles

Associated articles

Supplementary files

Article information

Article type
Paper
Submitted
15 May 2018
Accepted
24 Jul 2018
First published
02 Aug 2018

Lab Chip, 2018,18, 2653-2664

A flow focusing microfluidic device with an integrated Coulter particle counter for production, counting and size characterization of monodisperse microbubbles

J. M. R. Rickel, A. J. Dixon, A. L. Klibanov and J. A. Hossack, Lab Chip, 2018, 18, 2653 DOI: 10.1039/C8LC00496J

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