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Static droplet array for culturing single live adherent cells in an isolated chemical microenvironment

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Abstract

We present here a new method to easily and reliably generate an array of hundreds of dispersed nanoliter-volume semi-droplets for single-cells culture and analysis. The liquid segmentation step occurs directly in indexed traps by a tweezer-like mechanism and is stabilized by spatial confinement. Unlike common droplet-based techniques, the semi-droplet wets its surrounding trap walls thus supporting the culturing of both adherent and non-adherent cells. To eliminate cross-droplet cell migration and chemical cross-talk each semi-droplet is separated from a nearby trap by an ∼80 pL air plug. The overall setup and injection procedure takes less than 10 minutes, is insensitive to fabrication defects and supports cell recovery for downstream analysis. The method offers a new approach to easily capture, image and culture single cells in a chemically isolated microenvironment as a preliminary step towards high-throughput single-cell assays.

Graphical abstract: Static droplet array for culturing single live adherent cells in an isolated chemical microenvironment

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Publication details

The article was received on 17 Apr 2018, accepted on 13 Jun 2018 and first published on 16 Jun 2018


Article type: Paper
DOI: 10.1039/C8LC00403J
Citation: Lab Chip, 2018, Advance Article
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    Static droplet array for culturing single live adherent cells in an isolated chemical microenvironment

    A. Hassanzadeh-Barforoushi, A. M. K. Law, A. Hejri, M. Asadnia, C. J. Ormandy, D. Gallego-Ortega and M. Ebrahimi Warkiani, Lab Chip, 2018, Advance Article , DOI: 10.1039/C8LC00403J

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