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A fully-integrated and automated testing device for PCR-free viral nucleic acid detection in whole blood

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Abstract

Integrated devices for automated nucleic acid testing (NAT) are critical for infectious disease diagnosis to be performed outside of centralized laboratories. The gold standard methods for NAT are enzymatic amplification methods like the polymerase chain reaction that typically require expensive equipment and highly-trained personnel, limiting use in low-resource settings. A low-cost, integrated, rapid, portable and user-friendly point-of-care (POC) nucleic acid diagnostic device will improve the accessibility of NAT. Here, we present a fully integrated and simple-to-use POC device operated by a passive fluidic method that is able to perform a sequential multi-step assay to detect viral nucleic acids in blood. This simple device enabled the rapid detection of hepatitis C virus in blood in approximately 30 minutes with minimal sample handling by the user.

Graphical abstract: A fully-integrated and automated testing device for PCR-free viral nucleic acid detection in whole blood

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Publication details

The article was received on 09 Apr 2018, accepted on 29 May 2018 and first published on 29 May 2018


Article type: Paper
DOI: 10.1039/C8LC00371H
Citation: Lab Chip, 2018, Advance Article
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    A fully-integrated and automated testing device for PCR-free viral nucleic acid detection in whole blood

    W. Liu, J. Das, A. H. Mepham, C. R. Nemr, E. H. Sargent and S. O. Kelley, Lab Chip, 2018, Advance Article , DOI: 10.1039/C8LC00371H

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