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Roll-to-roll fabrication of integrated PDMS–paper microfluidics for nucleic acid amplification

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Abstract

Microfluidic-based integrated molecular diagnostic systems, which are automated, sensitive, specific, user-friendly, robust, rapid, easy-to-use, and portable, can revolutionize future medicine. Current research and development largely relies on polydimethylsiloxane (PDMS) to fabricate microfluidic devices. Since the transition from the proof-of-principle phase to clinical studies requires a vast number of integrated microfluidic devices, there is a need for a high-volume manufacturing method of silicone-based microfluidics. Here we present the first roll-to-roll (R2R) thermal imprinting method to fabricate integrated PDMS–paper microfluidics for molecular diagnostics, which allows production of tens of thousands of replicates in an hour. In order to validate the replicated molecular diagnostic platforms, on-chip amplification of viral ribonucleic acid (RNA) with loop-mediated isothermal amplification (LAMP) was demonstrated. These low-cost, rapid and accurate molecular diagnostic platforms will generate a wide range of applications in preventive personalized medicine, global healthcare, agriculture, food, environment, water monitoring, and global biosecurity.

Graphical abstract: Roll-to-roll fabrication of integrated PDMS–paper microfluidics for nucleic acid amplification

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Publication details

The article was received on 15 Mar 2018, accepted on 19 Apr 2018 and first published on 30 Apr 2018


Article type: Paper
DOI: 10.1039/C8LC00269J
Citation: Lab Chip, 2018, Advance Article
  • Open access: Creative Commons BY license
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    Roll-to-roll fabrication of integrated PDMS–paper microfluidics for nucleic acid amplification

    J. Hiltunen, C. Liedert, M. Hiltunen, O. Huttunen, J. Hiitola-Keinänen, S. Aikio, M. Harjanne, M. Kurkinen, L. Hakalahti and L. P. Lee, Lab Chip, 2018, Advance Article , DOI: 10.1039/C8LC00269J

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