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A universal platform for selection and high-resolution phenotypic screening of bacterial mutants using the nanowell slide

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Abstract

The Petri dish and microtiter plate are the golden standard for selection and screening of bacteria in microbiological research. To improve on the limited resolution and throughput of these methods, we developed a universal, user-friendly platform for selection and high-resolution phenotypic screening based on the nanowell slide. This miniaturized platform has an optimal ratio between throughput and assay complexity, holding 672 nanowells of 500 nl each. As monoclonality is essential in bacterial genetics, we used FACS to inoculate each nanowell with a single bacterium in 15 min. We further extended the protocol to select and sort only bacteria of interest from a mixed culture. We demonstrated this by isolating single transposon mutants generated by a custom-made transposon with dual selection for GFP fluorescence and kanamycin resistance. Optical compatibility of the nanowell slide enabled phenotypic screening of sorted mutants by spectrophotometric recording during incubation. By processing the absorbance data with our custom algorithm, a phenotypic screen for growth-associated mutations was performed. Alternatively, by processing fluorescence data, we detected metabolism-associated mutations, exemplified by a screen for β-galactosidase activity. Besides spectrophotometry, optical compatibility enabled us to perform microscopic analysis directly in the nanowells to screen for mutants with altered morphologies. Despite the miniaturized format, easy transition from nano- to macroscale cultures allowed retrieval of bacterial mutants for downstream genetic analysis, demonstrated here by a cloning-free single-primer PCR protocol. Taken together, our FACS-linked nanowell slide replaces manual selection of mutants on agar plates, and enables combined selection and phenotypic screening in a one-step process. The versatility of the nanowell slide, and the modular workflow built on mainstream technologies, makes our universal platform widely applicable in microbiological research.

Graphical abstract: A universal platform for selection and high-resolution phenotypic screening of bacterial mutants using the nanowell slide

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Publication details

The article was received on 15 Feb 2018, accepted on 07 May 2018 and first published on 08 May 2018


Article type: Paper
DOI: 10.1039/C8LC00190A
Citation: Lab Chip, 2018, Advance Article
  • Open access: Creative Commons BY-NC license
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    A universal platform for selection and high-resolution phenotypic screening of bacterial mutants using the nanowell slide

    H. Antypas, M. Veses-Garcia, E. Weibull, H. Andersson-Svahn and A. Richter-Dahlfors, Lab Chip, 2018, Advance Article , DOI: 10.1039/C8LC00190A

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