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Microstructure guided multi-scale liquid patterning on open surface


Liquid patterning is a quintessential aspect in cell-based screening. While there are a variety of methods to handle microliquids utilizing surface treatments, complex microfluidic system, and automated dispensing, most of the stated methods are both expensive and difficult to implement. Here, we present a fast multi-scale microliquid-patterning method on open surfaces using embossed microstructures without surface modification. Arrays of micropillars can trap microliquids when a bulk drop is swept by an elastic sweeper on polystyrene (PS) substrates. The patterning mechanism on basic form of 2X2 rectangular array of circular pillars is analyzed theoretically and verified with experiments. Nanoliter-to-microliter volume of liquids are patterned into various shapes by arranging pillars based on the analysis. Furthermore, an array of geometrically modified pillars can capture approximately 8,000 droplets on a large substrate (55 mm x 55 mm) in one step. Given the simplistic method of wipe patterning, the proposed platform can be utilized in both manual benchtop and automated settings. We will provide proof of concept experiments of single colony isolation using nanoliter-scale liquid patterning and of human angiogenic vessel formation using sequentail patterning of microliter-scale liquid.

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Publication details

The article was received on 01 Dec 2017, accepted on 04 May 2018 and first published on 16 May 2018

Article type: Paper
DOI: 10.1039/C7LC01288H
Citation: Lab Chip, 2018, Accepted Manuscript
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    Microstructure guided multi-scale liquid patterning on open surface

    D. Park, M. Kang, J. W. Choi, S. Paik, J. Ko, S. Lee, Y. Lee, K. Son, J. Ha, M. Choi, W. Park, H. Kim and N. L. Jeon, Lab Chip, 2018, Accepted Manuscript , DOI: 10.1039/C7LC01288H

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