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Proof-of-concept: Interfacing the liquid sampling-atmospheric pressure glow discharge ion source with a miniature quadrupole mass spectrometer towards trace metal analysis in cell culture media

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Abstract

In an effort to provide a mass spectrometry system capable of at-reactor trace metal analysis of bioprocess media and clarified cell culture fluid, the liquid sampling-atmospheric pressure glow discharge (LS-APGD) ion source was interfaced with a Waters QDa mass spectrometer. The LS-APGD is capable of elemental analysis, as well as organic compound determinations. The Waters QDa is a compact, rack-mounted single quadrupole mass analyzer commonly employed in an integrated liquid chromatography system. By replacing the QDa's standard electrospray ionization (ESI) source with the LS-APGD, trace metal analysis of bioprocess stock media samples can be performed, alleviating use of high-end, inductively coupled plasma (ICP-MS) instruments. Presented here is a proof-of-concept effort, interfacing the microplasma to this platform for the first time. Preliminary optimization of the LS-APGD operating parameters and the QDa's in-source collision induced dissociation (CID) conditions was performed, yielding signal intensities of >6 × 107 AU for a multi-element test solution containing 25 μg mL−1 of Cu, Ag, and Tl. As further proof-of-concept, Chinese hamster ovary (CHO) cell culture medium was spiked with the same elemental concentrations and analyzed on the LS-APGD/QDa system. Stable plasma response allows spectral background subtraction of the media components, yielding analytically-relevant elemental signals. These results suggest that the LS-APGD/QDa coupling may be a viable approach for at-bioreactor, elemental analysis.

Graphical abstract: Proof-of-concept: Interfacing the liquid sampling-atmospheric pressure glow discharge ion source with a miniature quadrupole mass spectrometer towards trace metal analysis in cell culture media

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Publication details

The article was received on 20 Aug 2018, accepted on 06 Sep 2018 and first published on 14 Sep 2018


Article type: Technical Note
DOI: 10.1039/C8JA00278A
Citation: J. Anal. At. Spectrom., 2018, Advance Article
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    Proof-of-concept: Interfacing the liquid sampling-atmospheric pressure glow discharge ion source with a miniature quadrupole mass spectrometer towards trace metal analysis in cell culture media

    E. D. Hoegg, B. A. Patel, William N. Napoli, D. D. Richardson and R. K. Marcus, J. Anal. At. Spectrom., 2018, Advance Article , DOI: 10.1039/C8JA00278A

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