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Direct immunoassays and their performance – theoretical modelling of the effects of antibody orientation and associated kinetics

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Abstract

The orientation and activity of antibodies immobilized on solid surfaces are of direct relevance to many immunosensing applications. We therefore investigate a mathematical model which estimates the fraction of antibodies which are available for reaction in a randomly adsorbed sample. Numerical simulations are presented which highlight the separate effects of antibody orientation, accessibility and loss of binding ability on the amount of captured antigen. The assay response can then be expressed as a function of total antibody density and used for optimizing the surface coverage strategy under various conditions.

Graphical abstract: Direct immunoassays and their performance – theoretical modelling of the effects of antibody orientation and associated kinetics

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Publication details

The article was received on 02 May 2018, accepted on 30 Aug 2018 and first published on 30 Aug 2018


Article type: Paper
DOI: 10.1039/C8IB00077H
Citation: Integr. Biol., 2018, Advance Article

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