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Issue 31, 2018
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Impact of the lanthanide contraction on the activity of a lanthanide-dependent methanol dehydrogenase – a kinetic and DFT study

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Abstract

Interest in the bioinorganic chemistry of lanthanides is growing rapidly as more and more lanthanide-dependent bacteria are being discovered. Especially the earlier lanthanides have been shown to be preferentially utilized by bacteria that need these Lewis acids as cofactors in their alcohol dehydrogenase enzymes. Here, we investigate the impact of the lanthanide ions lanthanum(III) to lutetium(III) (excluding Pm) on the catalytic parameters (vmax, KM, kcat/KM) of a methanol dehydrogenase (MDH) isolated from Methylacidiphilum fumariolicum SolV. Kinetic experiments and DFT calculations were used to discuss why only the earlier lanthanides (La–Gd) promote high MDH activity. Impact of Lewis acidity, coordination number preferences, stability constants and other properties that are a direct result of the lanthanide contraction are discussed in light of the two proposed mechanisms for MDH.

Graphical abstract: Impact of the lanthanide contraction on the activity of a lanthanide-dependent methanol dehydrogenase – a kinetic and DFT study

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Publication details

The article was received on 29 Mar 2018, accepted on 14 Jun 2018 and first published on 18 Jul 2018


Article type: Paper
DOI: 10.1039/C8DT01238E
Citation: Dalton Trans., 2018,47, 10463-10472
  • Open access: Creative Commons BY-NC license
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    Impact of the lanthanide contraction on the activity of a lanthanide-dependent methanol dehydrogenase – a kinetic and DFT study

    H. Lumpe, A. Pol, H. J. M. Op den Camp and L. J. Daumann, Dalton Trans., 2018, 47, 10463
    DOI: 10.1039/C8DT01238E

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